The World Health Organization (WHO) has set at least 70% PCR confirmation rate as target for countries to meet, yet most countries in the African region have not been able to reach this target. Rather, the rate of confirmation has been declining; and this is due to, amongst other factors, ambiguity in PCR testing protocols in a number of these endemic countries
Hence, to resolve procedure ambiguity issues and to ensure sustainability, testing protocols were successfully harmonized and standardized during the first meeting in October 2019. Every laboratory in the network would use the same testing procedure and same reagents for PCR diagnosis.
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BU-LABNET PCR SOP 1
Standard Operating Protocol 1 : Recommendation for sampling, transport and storage of samples for Buruli ulcer diagnosis With a worksheet for…
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BU-LABNET PCR SOP 2
Standard Operating Protocol 2 : Registration and Treatment of samples prior to PCR analysis for Buruli ulcer
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BU-LABNET PCR SOP 3 with IPC
Standard Operating Protocol 3 : Extraction and Purification of DNA from M. ulcerans (with Internal Positive Control)
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BU-LABNET PCR SOP 3 without IPC
Standard Operating Protocol 3: Extraction and Purification of DNA from M. ulcerans (without Internal Positive Control).
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BU-LABNET PCR SOP 4 with IPC
Standard Operating Protocol 4 : Real-time Polymerase Chain Reaction (QPCR) preparation with Internal Positive Control
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